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American Society of Microbiology ABMM Sample Questions:

1. During the investigation of a foodborne illness outbreak linked to raw sprouts, several bacterial isolates are obtained from patient stool samples and the implicated food source. Which of the following molecular methods would be MOST useful for establishing a definitive link between the clinical isolates and the food isolate?

A) Gram staining and biochemical profiling
B) Antimicrobial susceptibility testing
C) Serotyping based on O and H antigens
D) Pulsed-field gel electrophoresis (PFGE)

2. Microscopic examination of a potassium hydroxide (KOH) preparation of skin scrapings from a patient with tinea versicolor typically reveals:

A) Septate hyphae and arthroconidia
B) Cigar-shaped yeast cells
C) Broad, aseptate hyphae with sporangiophores
D) Short, unbranched hyphae and globose yeast cells in clusters ("spaghetti and meatballs")

3. A patient with HIV infection and a CD4+ T-cell count below 100 cells/µL presents with headache, fever, and altered mental status. CSF analysis reveals elevated protein and low glucose. India ink stain is negative.
Cryptococcal antigen test of the CSF is positive. The MOST appropriate initial treatment is:

A) Voriconazole
B) Amphotericin B and flucytosine
C) Caspofungin
D) Fluconazole

4. A microbiology laboratory is performing Gram staining on a bacterial smear. If the safranin counterstain is omitted, Gram-negative bacteria will appear:

A) Brown
B) Purple
C) Blue
D) Colorless

5. A serum sample from an asymptomatic patient screened for syphilis yields a reactive Rapid Plasma Reagin (RPR) test result with a titer of 1:16, but a non-reactive Treponema pallidum particle agglutination (TP-PA) test. What is the most likely interpretation of these findings?

A) Early primary syphilis (before treponemal antibodies develop)
B) Biological false positive (BFP) RPR reaction
C) Prozone phenomenon in the TP-PA test
D) Successfully treated past syphilis infection

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